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je pensais que c’est le gon-miiga ou le gon-pelga qui en .. Phusion® High-Fidelity DNA Polymerase | NEB. Manufactured and quality-controlled at New England Biolabs, Thermo Scientific ® Phusion High-Fidelity DNA Polymerase offers both high fidelity and robust performance. 50X higher fidelity than Taq. Robust reactions - maximal success with minimal optimization. Offered with multiple buffers for customized reaction setup for different DNA templates.. NEBuilder® HiFi DNA Assembly Master Mix | Gene Assembly | NEB. Transformation: NEB 5-alpha Competent E. coli (High Efficiency, NEB #C2987) provided with the NEBuilder HiFi DNA Assembly Cloning Kit are recommended for use for assembled products of less than 15kb. It is also possible to use other NEB competent E. coli strains, with the exception of BL21, BL21(DE3), Lemo21(DE3), Nico(DE3), and SHuffle®.. Research | NEB. Research at NEB. At NEB, basic research is a priority. Well established in their respective fields, including Molecular Biology and Parasitology, NEB scientists routinely publish their work in the leading journals, present research at scientific conferences, host visiting scholars from around the world, and supervise post-doctoral fellows.. NEBcloner. Traditional Cloning Workflows. Select a workflow step below to determine recommended products and protocols. Use NEBcloner to find the right products and protocols for each in your traditional cloning workflow, including double digestion buffers.. Neb Medical: Breast Pumps and Nebulizers Covered by Insurance. Neb Medical is an accredited, durable medical equipment company. We have worked with thousands of satisfied customers, helping them save money while also getting them top-line medical equipment. We also partner with hundreds of physicians and hospitals. Our philosophy is that every patient deserves top-quality treatment, whether you get your .. Ligation Protocol with T4 DNA Ligase (M0202) | NEB. Insert DNA (1 kb) 37.5 ng (0.060 pmol) Nuclease-free water. to 20 μl. T4 DNA Ligase